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ISO 8128-2:1993 Apple juice, apple juice concentrates and drinks containing apple juice -- Determination of patulin content -- Part 2: Method using thin-layer chromatography
standard by International Organization for Standardization, 07/01/1993
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The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothiazoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 g/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.