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standard by International Organization for Standardization, 12/01/1993
The method described is based on placing adult earthworms in a defined substrate containing the test substance in different concentrations and determining the percent mortality after 7 d and 14 d. It is not applicable to volatile substances, i.e. substances for which Henry's constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,0133 Pa at 25 C. It does not take into account the possible degradation of the test substance.